site stats

Mcherry-gfp-lc3实验步骤

Web2 jun. 2011 · A tandem fusion of mCherry and GFP is fused to LC3B (one of the several members of the mammalian LC3 family) to make a pH-sensitive sensor that is used to … WebC3003-100μl. Lenti-EGFP-LC3B (10^9TU/ml) 100μl. 2992.00元. Lenti-EGFP-LC3B，即Lentivirus expressing EGFP-LC3B fusion protein，是一种可以表达EGFP-LC3B融合蛋白的慢病毒，可以用于感染细胞或组织后进行细胞自噬 (autophagy)检测。. 本产品带有嘌呤霉素 (puromycin)抗性，感染细胞后可以筛选 ...

LC3双荧光自噬慢病毒的应用（上篇）——实验原理及病毒使用

Web(1) an inhibitor of macropinocytosis upregulation, wherein the inhibitor of macropinocytosis upregulation is selected from 5-(N-ethyl-N-isopropyl) amiloride (EIPA), 3-methylsulpho WebHepG2 cells were transduced with mCherry-GFP-LC3 lentiviral vectors, which were pre-packed with the lentivirus packaging kit. The quenching properties of the GFP fluorescent signal in acid conditions (pH < 5) and the cherry fluorescent signal do not differ significantly in different acidic environments [ 28 ] when used to detect autophagic flux. christmas lights went out on tree https://bonnesfamily.net

背景更低的红色荧光蛋白—mCherry - 知乎 - 知乎专栏

Web10 apr. 2024 · Main. Macro-autophagy (hereafter termed ‘autophagy’) encapsulates large intracellular cargos in a double-membrane structure named the autophagosome 1, 2. Upon fusion of the autophagosome with ... Web具体实验流程如下： 1）Day1：细胞感染 细胞感染过表达 SensGFP-StubRFP-LC3 的自噬检测慢病毒（注：自噬检测慢病毒具有 shRNA 表达框，因此可同时在此步骤对待研基因进 … WebK. Castillo, ... C. Hetz, in Methods in Enzymology, 2024 2.1 Design of AAV2_mCherry-GFP-LC3 Vectors. The whole mCherry-GFP-LC3 expression cassette was excised from a plasmid kindly provided by Terje Johansen's laboratory, and inserted into a shuttle proviral plasmid, containing both the AAV2/2 inverted terminal repeats and the 1.6-kb … get body in shape one month from vacation

Autophagy restricts Mycobacterium tuberculosis during acute …

Category:Lenti-EGFP-LC3B (10^9TU/ml)(EGFP-LC3B慢病毒)(C3003-100μl)

Tags:Mcherry-gfp-lc3实验步骤

Mcherry-gfp-lc3实验步骤

Methods in Mammalian Autophagy Research - PMC - National …

Web按照步骤1.获得的条件进行感染实验，适当条件诱导自噬 (如EBSS或者细胞自噬激活剂处理等)，随后可在荧光显微镜下观察LC3B的荧光变化情况。 附录参考： 腺病毒使用安全规 … WebPlasmid pBABE-puro mCherry-EGFP-LC3B from Dr. Jayanta Debnath's lab contains the insert microtubule-associated protein 1 light chain 3 beta and is published in EMBO Rep. 2009 Feb;10(2):173-9. doi: 10.1038/embor.2008.238. Epub 2009 Jan 16. This plasmid is available through Addgene.

Mcherry-gfp-lc3实验步骤

Did you know?

WebmCherry-GFP-LC3 Cloning Information Cloning method TOPO Cloning 5′ sequencing primer CGCGGATCCGGTCGCCACCATGGTGAGCAAGGGCGAG 3′ sequencing primer … WebTo explain our findings, GFP-LC3 and GFP-RFP-LC3 adenoviruses and autophagy regulators were used, and the results suggested that doxazosin blocked autophagy in HSCs in vitro. To further confirm our findings, an mCherry-EGFP-LC3 adeno-associated virus was administered to mice, and the results showed that doxazosin suppressed autophagy in …

WebLC3蛋白合成后被Atg4剪切掉C端5肽，暴露甘氨酸残基，产生胞浆定位的LC3-I。 在自噬过程中，LC3-I会被包括Atg7和Atg3在内的泛素样体系修饰和加工，与磷脂酰乙醇胺（PE） … WebLC3 is a commonly used marker for measuring autophagic flux. mRFP-GFP-LC3 functions as an autophagy bionsensor via the same mechanism as Rosella: GFP is quenched in acidic environments, while mRFP is relatively stable and fluorescences even at the acidic pH found in lysosomes.

WebPlasmid pDEST-CMV mCherry-GFP-LC3B WT from Dr. Robin Ketteler's lab contains the insert Microtubule-associated proteins 1A/1B light chain 3B and is published in Autophagy. 2024 Jan 21. doi: 10.1080/15548627.2024.1569925. This plasmid … Web24 okt. 2024 · Careful characterization using LysoTracker and mCherry-LC3 transgenic fish demonstrated this was due to a block in autophagosome degradation rather than an increase in autophagosome formation. A dual GFP-LC3; mCherry-Lamp1 reporter line recently developed by the same group was used to further elucidate the role of lysosome ...

WebAd-mCherry-GFP-LC3B，即adenovirus expressing mCherry-GFP-LC3B fusion protein，是一种可以表达mCherry-GFP-LC3B融合蛋白的腺病毒，可以用于感染细胞或 …

WebTandem fluorescent-tagged LC3 (mRFP-EGFP-LC3) is a convenient assay for monitoring autophagic flux based on different pH stability of EGFP and mRFP fluorescent proteins. … get body odor out of couchWeb14 nov. 2013 · The whole mCherry-GFP-LC3 expression cassette was excised from the plasmid and inserted into a shuttle pro-viral plasmid, containing both the AAV2/2 inverted terminal repeats (ITRs) and the 1.6-kb ... get body into ketosis fastWeb① 利用Western Blot检测LC3-II/I比值的变化以评价细胞自噬形成（图4）。 细胞自噬形成时，胞浆型LC3-I会酶解掉一小段多肽，随后跟PE结合转变为膜型的LC3-II。 因此可以通 … christmas lights west midlandsWeb从结构上看突变体mcherry能够具有如此强的竞争力有2个原因。 1，mcherry中的第163位Lys已经突变成了Gln，这个突变的氨基酸所在的侧链位于发色团苯环下方，在结构中能够清楚的看到163位的Lys与发色团 … christmas lights wells maineWeb,南模生物是国内模式生物领域领先的生物科技公司，专业从事遗传修饰动物模型(转基因小鼠、斑马鱼、线虫)的研发、饲养 ... christmas lights west palm beach flhttp://www.geneppl.com/productinfo.php?id=680014 christmas lights white backgroundWebpCMV-GFP-LC3B是碧云天自行研发的在哺乳动物细胞中表达GFP标签的人源LC3B 融合蛋白的质粒。该质粒含有CMV启动子，为卡那霉素抗性，转染细胞后能够在靶细胞中高效表达绿色荧光蛋白GFP标签的LC3B的融合蛋白，呈现明亮的绿色荧光，可以用于细胞自噬(autophagy)的研究。 get body of email python